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- 產(chǎn)品名稱(chēng)： Anti-DNA-RNA Hybrid [S9.6] Antibody
- 產(chǎn)品貨號(hào)： CS2112
- 貨期： 現(xiàn)貨
- 價(jià)格與訂購(gòu)： 2800
- 數(shù)量：
庫(kù)存： 100
- 規(guī)格： 100μl
- 產(chǎn)品信息
- 如何訂購(gòu)
Synonyms
DNA-RNA hybrid; RNA/DNA hybrid
Attribute
Mouse Monoclonal Antibody
Isotype
Mouse IgG2a
Purity
Protein A/G Purification
Application
ELISA, IP, IF, CHIP, Dot Blot
Immunogen
ΦX174 bacteriophage-derived synthetic DNA/RNA
Buffer
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Storage
Store at -20℃. Do not aliquot
Recommended Dilution
IP: 1:50-500IF: 1: 200
Background
The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity.
Immunofluorescentanalysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500 and Alexa Fluor 647- conjugatedAffiniPure Goat Anti-Mouse IgG(H+L)
Chromatin immunoprecipitationanalysis of HeLa cells
genomic DNA(gDNA)using DNA-RNA hybrid Mouse Monoclonal
Antibody [S9.6] at dilution of 1:200
Note
For research use only .
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產(chǎn)品銷(xiāo)售：
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Amily
