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天然產(chǎn)物 > 生物堿

Capsaicin ((E)-Capsaicin) 屬于天然產(chǎn)物，提取自辣椒，是一種 TRPV1 激動劑 (EC50=0.29 μM)。Capsaicin 具有抗腫瘤、抗炎、抗氧化、神經(jīng)保護等活性。

TRPV1 (HEK293 cells):0.29 μM(EC50)

404-86-4

Capsaicin is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. FaDu cells are plated at a density of 1×105 cells/well on 24-well plate. After overnight growth, the cells are treated with various concentrations of Capsaicin (0 μM, 50 μM, 100 μM, 150 μM, 200 μM, 250 μM, 300 μM, and 350 μM) for 24, 48 and 72 hours, with medium replacement every 24 hours. At the end of treatment, 30 μL of the tetrazolium compound MTT, and 270 μL of fresh medium are added. After further incubation for 4 hours at 37°C, 200 μL of 0.1 N HCl in 10% SDS is added into each well to dissolve the tetrazolium crystals. Finally, the absorbance at a wavelength of 540 nm is recorded using an ELISA plate reader[1].

Qutenza, 8-Methyl-N-vanillyl-trans-6-nonenamide, 天然辣椒素, Vanilloid, 辣椒素, (E)-Capsaicin, Zostrix

>99.41%

C₁₈H₂₇NO₃

305.41

DMSO: 50 mg/mL (163.71 mM)