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- 產品名稱： Rab7 Activation Assay Kit
- 產品貨號： 82501
- 貨期： 現(xiàn)貨
- 價格與訂購： 6800
- 數量：
庫存： 5
- 規(guī)格： 96T
- 產品信息
- 如何訂購
Product Description
Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome,cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.
Currently there is no direct assay to measure the activation of Rab7 GTPases.
Bioyears Biosciences Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7 GTP, but not Rab7 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Currently there is no direct assay to measure the activation of Rab7 GTPases.
Bioyears Biosciences Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7 GTP, but not Rab7 GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab7-GTP monoclonal antibodies can also be used to monitor the activation of Rab7 in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab7 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab7. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Rab7 Activation Assay Kit bases on the configuration-specific anti Rab7 GTP monoclonal antibody to measure the active Rab7 GTP levels, either from cell extracts or from in vitro GTPγS loading Rab7 activation assays. Briefly, anti active Rab7 mouse monoclonal antibody will be incubated with cell lysates containing Rab7 GTP. The bound active Rab7 will then be pulled down by protein A/G agarose. The precipitated active Rab7 will be detected by immunoblot analysis using antiRab7 rabbit polyclonal antibody.
Kit Components
1. Anti active Rab7, Mouse Monoclonal Antibody (Catalog No. 26923): One vial – 22 μL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab7 GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750 mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti Rab7, Rabbit Polyclonal Antibody (Catalog No. 21069): One vial – 100 μL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 ?l at 10 mM, use 5 μL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 ?l at 100 mM, use 5 μL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4oC until their expiration dates.
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Note
For Research Use Only.
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