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- 產(chǎn)品名稱： Anti RAS(G13R) Mouse Monoclonal Antibody
- 產(chǎn)品貨號： CST26474
- 貨期： 現(xiàn)貨
- 價格與訂購： 4800
- 數(shù)量：
庫存： 5
- 規(guī)格： 100μg
- 產(chǎn)品信息
- 如何訂購
Catalog Number
CST26474
Gene Symbol
H-Ras; K-Ras; N-Ras
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Description
Anti RAS(G13R) Mouse Monoclonal Antibody
Background
Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen
A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes G13R mutant, but not wild-type RAS of vertebrates.
Immunoprecipitation/Western Blot
Western blot analysis of recombinant HRAS(G13R) and wildtype proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with Anti RAS(G13R) monoclonal antibody (Cat. #26474).
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Immunofluorescence:
Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid , pCDNA3-GFP Ras(G13C) plasmid , pCDNA3-GFP Ras(G13V) plasmid , pCDNA3-GFP Ras(G13A) plasmid , or pCDNA3-GFP Ras(G13D) plasmid , then fixed and stained with Anti RAS(G13R) monoclonal antibody (Cat. #26474).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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