国产精品国产一页_亚洲爆乳精品无码一区二区_黄色影片一级毛片_久久99国产精品免费观看_日韩av中美av中文字幕

159-2642-3062 027-65317797
服務(wù)熱線(工作日:9:00-17:30)

瀏覽量: 303

  • 產(chǎn)品名稱: Cy5 Alkyne
  • 產(chǎn)品貨號: CSC313
  • 貨期: 現(xiàn)貨
  • 價(jià)格與訂購: 1800
  • 數(shù)量:
    庫存: 10
  • 規(guī)格: 1 µmol
  • 產(chǎn)品信息
  • 如何訂購
    Introduction
    Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
    The Cy5 alkyne is reactive with azide via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.
    Features
    Efficiency—the click reaction is complete in less than 1 hour;
    Specificity—the reaction between the label and detection tag is selective and specific;
    Stability—the reaction product contains an irreversible, covalent bond;
    Biologically inert—the components of the reaction do not undergo any side reactions.
    Figure 1. Click chemistry labeling
    Label
    Cy5
    Ex/Em
    650/665
    Detection Method
    Fluorescent 
    Solubility
    DMSO, DMF
    Molecular Weight
    913.20
    Product Size
    1 μmol 
    Storage Conditions
    -20 ℃, protect from light 
    Shipping Condition
    Room Temperature 
    Applications
    Click chemistry labeling 
    Reference
    1.Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
    Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,Angew Chem Int Ed Engl (2012) 51:3143-3146
    2.Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.
    Winz ML, Samanta A, Benzinger D, J?schke A,Nucleic Acids Res (2012) 40:e78-e78
    3.Mutant methionyl-tRNA synthetase from bacteria enables site-selective N-terminal labeling of proteins expressed in mammalian cells.
    Ngo JT, Schuman EM, Tirrell DA,Proc Natl Acad Sci U S A (2013) 110:4992-4997
    4.Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
    Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,J Am Chem Soc (2008) 130:11576-11577
    5.Robust fluorescent detection of protein fatty-acylation with chemical reporters.
    Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,J Am Chem Soc (2009) 131:4967-4975
    Note
    For research use only .
岑溪市| 鄂伦春自治旗| 信宜市| 海伦市| 泗洪县| 大足县| 大悟县| 普兰店市| 永宁县| 珲春市| 乌鲁木齐县| 乌兰县| 通道| 清水县| 澳门| 祁阳县| 班戈县| 苏尼特右旗| 报价| 灵川县| 大兴区| 蒲江县| 沾化县| 广昌县| 平乐县| 稻城县| 东丰县| 西峡县| 昌邑市| 商城县| 红原县| 永平县| 涟源市| 宝兴县| 丹棱县| 龙游县| 揭东县| 河津市| 察雅县| 松原市| 宜阳县|